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2. Al-Baqarah/The Cow

2. Al-Baqarah/The Cow

I/We begin by the Blessed Name of Allah

The Immensely Merciful to all, The Infinitely Compassionate to everyone.

02:01
a. Alif. Lam. Mim.

02:02
This Qur’an is the awaited Divine Book.
Let there be no doubt in that this is the Absolute Word of Allah in Arabic and no one can ever change it.
This is a manifesto that provides guidance for those who endeavor to shape their lives as well as their social behavior in a manner that seeks Allah’s Pleasure: the ever Allah- reverent.

02:03
They are the people who
believe in whatever is beyond their senses and perception, and
establish the Salat /Prayers and
spend a good part of their wealth which WE have favored them with in the Cause of Allah, human development, and community welfare.

02:04
And they also believe in that which is being sent down on to you O The Prophet,
and all Divine Scriptures that were sent down before you on to the earlier Prophets and
they firmly believe in the All-embracing concept of the Hereafter and its correlatives.

02:05
Thus, it is they who are living by the Guidance from their Rabb - The Lord.
And it is also they who are going to be successful.

02:06
Surely those who willfully deny and belie the truth of these beliefs despite your advocacy,
it is a matter of gross indifference to them whether you warn them of the consequences of disbelief or do not warn them, they are not going to believe.

02:07
Allah has set a seal upon
their hearts, and
their hearing, and
over their intellectual vision...

The Importance of Emerging Sciences & Technology in the Light of Quran & Prophetic Teachings

Science and technology is on top position contemporary now it is need of our masses to compare these formulas with holy Quran because Quran is miracle and implementable till the day of  Judge ment  lot of scientists have benefited by Quran and they also agree up on the excellence of Quran. Hence in this research we have to prove dominancy of Quran over on the science and technology the hints of new inventions and discoveries are available in Quran if we study the Quran deeply we will find in it complete agreement with modern science however it was revealed in that time people were not aware from scientific inventions and they have not know-how of it. Comparatively this time is well advanced period and lot of things invented while concept of these things was present in this sky book I have drawn intention of readers on these points. In this research I would discussed that importance of science and technology specially the sub subjects of the modern science math and statistics geology, astronomy, space sciences, Embryology, Genetics and computer technology. It should be remember that the exposition of this book is also two types scientifically and logically because Quran invites us towards intelligence and wisdom.   It is responsibility of scholars to interpret the Quran contemporary because this is not merely ancient book but it is for all times and not for Arabs but for guide line of whole humanity of entire universe. It is challenges itself that no any kind of error or mistake in it foes of Islam tried to find faults but they not succeeded to do so resulting they affirmed dignity of it over on all the books.

Cloning and Transformation of Sucrose Synthase Sus Gene in Cotton Gossypium Hirsutum L.

Long staple length, optimum micronaire with good strength is the highest demand of the textile industry in Pakistan. Cotton fiber quality is a multifactorial trait controlled by different genes that play a decisive role in different stages of fiber development. However, only a few genes have been explored yet that are crucial in fiber development and some of them have shown a positive effect on fiber yield and quality in transgenic cotton. Sucrose synthase (SuS) is among the important factors involved in inter-conversion of sucrose to fructose and UDP-glucose that serve as a precursor for the synthesis of cell wall cellulose. In the current study, an attempt was made by the introduction of synthetic SuS gene under the control of a CaMV35S promoter. The cassette was cloned in pCAMBIA 1301 vector with BstX1 and Xho1 restriction sites. The recombinant plant expression vector pCAMBIA-SuS was introduced into local non-transgenic cotton (Gossypium hirsutum) variety CEMB-00 through Agrobacterium-mediated transformation method. The transformation efficiency in this study remained 1.02%. Molecular analyses of putative transgenic cotton plants were performed to verify their transgenic nature. Young leaves of T0 plants were used for histochemical GUS assay to confirm the transformation event. Amplification of 813bp fragment by using gene-specific internal detection primers confirmed the successful integration of SuS gene in the cotton genome. Maximum mRNA expression of SuS gene was obtained in MA0023 and MA0034 as compared to other transgenic cotton plants. Four transgenic plants were selected after PCR analysis for molecular and biochemical studies in advanced generations. SuS gene mRNA expression in fiber was significantly higher at 15 DPA and 20 DPA as compared to 8 DPA in all transgenic lines. The Leaf SuS activity in transgenic lines increased up to 54% as in the case of MA0023. SuS activity at the reproductive stage (15DPA) was also increased up to 40% in case of MA0034. In fiber of transgenic lines decrease in sucrose contents while the increase in total soluble sugars contents was evident from elongation (15DPA) towards secondary wall synthesis and maturation (40DPA). Cleavage of sucrose by SuS catalytic activity was found to be higher in transgenic cotton plants as compared to non-transgenic control plants. The mRNA expression was found positively correlated with SuS activity and cellulose contents. Fiber analyses of transgenic plants showed an increase in fiber length up to 11.7%, fiber strength 18.65% and 28% increase in cellulose contents. Improvement in micronaire value up to 3.00 was observed in transgenic line MA0023. SEM analyses revealed that fibers from transgenic plants were smooth, highly spiral and fiber twist number increased per unit length when compared with control. Improvement in fiber length, micronaire value and surface smoothness led to producing better quality fiber for textile purposes. Genetic modification of cotton with SuS gene also resulted in improvement in some morphological and agronomic traits like plant height increased up to 88.76%, number of bolls 87.80% and GOT increased up to 22.08 % as compared to control plants. FISH analysis showed single copy number at chromosome number 9 and no signal was detected in control plants. The results depict that increased SuS activity, fiber properties, morphological and agronomic traits in transgenic lines are caused by genome integration and constitutive expression of SuS gene in cotton. From the results, it is clear that the SuS gene holds the potential to improve fiber quality and also provide more positive effect when combined with other potential fiber trait. Stacking of fiber-related genes into a single plant could be a better way to improve fiber quality in local cotton varieties to meet the standards of the modern textile industry.
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