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صبح قفس کا جمالیاتی پہلو

صبحِ قفس کا جمالیاتی پہلو
سر زمین پاک پتن کی عظمت ورفعت کے سے کیسے انکار ہو سکتا ہے۔ یہی وہ مخزن ومعدن تصوف ہے جس میں پائے جانے والے لعل وگہر کسی کو مفلوک الحال یا تشنہ لب نہیں رہنے دیتے۔ فیوض وبرکات سے مالامال اس خطہ تحریم سے محبت کرنے والا کبھی کسی احساس محرومی کاشکار نہیں رہ سکتا۔ خواہ وہ اس سے ہزاروں میل دور ہی کیوں نہ ہو مگر جو سعادت منداپنے صبح و مسا دامن حضرت گنج شکرؒ کے ساتھ وابستگی میں گزاررہا ہو یقینا اس کا طائر تخیل اوج سماء کی جانب ہر وقت محو پرواز رہتا ہے۔ تائب نظامی انھی خوش مقدر لوگوں میں شمار ہوتے ہیں جو ہر لمحہ مزار گنج شکرؒ کی تابش سے اپنے دل ونگاہ کو منور کیے ہوئے ہیں اور پھر خوش قسمتی سے اگر انسان شاعر اور ادیب بھی ہو تو یہاں قیام کا لطف دوآتشہ بلکہ سہ آتشہ ہو جاتا ہے۔ میں دل کی گہرائیوں سے یہ محسوس کرتا ہوں کہ صاحب مزارؒ کے تلطف اور نوازش سے تائب نظامی کے شعری وادبی حوصلوں کو نیاولولہ اور عزائم کو تخلیق کے نئے اُفق عطا ہوتے ہیں۔ صبحِ قفس’’عروض‘‘ کی ایک ایسی دلآویز ہے جو ’’گلہائے رنگارنگ سے ہے زینت چمن‘‘ کا دل پذیر منظر پیش کر رہی ہے۔ میں اس حقیقت کا اظہار کسی تصنع یاریا کے بغیر کر رہا ہوں کہ قدرتِ کاملہ نے اس ’’تلمیذخاص‘‘ کو ردیف قافیہ ، اوزان و بحور اور بندش الفاظ پر جو دسترس عطا کر رکھی ہے اس کی داد میرے امکان میں نہیں۔
غزلیات پر مشتمل صبح قفس کا آغاز حسب روایت حمدو نعت سے ہوتا ہے۔ شاعر ان اصناف کی نزاکتوں سے کماحقہ آگاہ ہیں۔ وہ حمد و نعت کی گہرائی وگیرائی سے اچھی طرح شناسا ہیں۔ حمد کا ایک شعر الوہیت رب...

Twenty-First Century Regionalism Impact on South Asia

The paper is an attempt to review twenty-first century regionalism and its impact on South Asia, which includes the rising role of China and Pakistan’s pivot status as contributing factors to peace, growth, and development. This coincides with a more south-oriented world. The theory applied is NeoFunctionalism, which explains the European integration and may be used to explain the potential modern South Asian integration process with China as a pre-cursor. This will perhaps be a positive outcome of the twenty-first century regionalism. The paper dwells upon intra-regional integration, sighting Eurasian model of connectivity as an example, and how it can be a role-model for developing countries. The emphasis remains on improved relations between Pakistan and India as a pre-requisite for regionalism to take off in South Asia.

Effects of Carbohydrate Binding Modules on Characteristics of Xylanases from Thermophilic Bacteria

Xylanases degrade the hemicellulosic component of plant biomass and find potential applications in poultry, paper, textile and biofuel industries. In this study, a novel, family GH10 enzyme, Xyn10B.CB3B2 from Acidothermus cellulolyticus 11B was characterized. This enzyme was found to be a trifunctional enzyme having endo xylanase, arabinofuranosidase and acetyl xylan esterase activities. Native xylanase, Xyn10B.CB3B2 had carbohydrate binding modules (CBM), CBM3 and CBM2 in tandem at the C-terminus. CBMs are protein domains that bind carbohydrate ligands and are found in carbohydrate active enzymes. Truncation of CBM2 was done to create Xyn10B.CB3 while CBM3 was fused to N-terminus of catalytic domain to form Xyn10B.B3C. Fusion of CBM2 at the C- and N-termini of the catalytic domain resulted in Xyn10B.CB2 and Xyn10B.B2C, respectively. In addition, only the catalytic domain (Xyn10B.C) was also characterized in this study. All of the enzyme variants were successfully expressed in soluble fraction of Escherichia coli cells and purified through binding with regenerated amorphous cellulose except Xyn10B.C that was obtained as inclusion bodies and purified by refolding. Activities of Xyn10B.CB3B2, Xyn10B.CB3, Xyn10B.B3C, Xyn10B.CB2, Xyn10B.B2C and Xyn10B.C on beechwood xylan were 118,305, 68,325, 65,825, 49,261, 44,518 and 40,368 U/μmol, respectively. Activities of Xyn10B.CB3B2, Xyn10B.CB3, Xyn10B.B3C, Xyn10B.CB2, Xyn10B.B2C and Xyn10B.C towards p nitorphenylarabinofuranoside were 9,042, 4,532, 4,026, 5,672, 5,137 and 4,340 U/μmol, respectively. Activities of Xyn10B.CB3B2, Xyn10B.CB3, Xyn10B.B3C, Xyn10B.CB2, Xyn10B.B2C and Xyn10B.C towards p-nitrophenylacetate were 15,545, 10,485, 8,856, 7,820, 7,571 and 7,342 U/μmol, respectively. All of the enzyme variants had optimum temperature 70 °C and optimum pH 6.0, under the vii assay conditions used. However, Xyn10B.C had optimum temperature and pH of 60 °C and 5.0-6.0, respectively. Binding assays revealed that all of the variants bound to insoluble oat spelt xylan and Avicel expect Xyn10B.C that did not bind to Avicel. Incubation of all enzyme variants with Mn2+ had negative impact on the activity of enzymes while other metal ions had no effect on the activity. Xyn10B.CB3B2 was stable up to 70 °C while Xyn10B.CB3, Xyn10B.B3C, Xyn10B.CB2 and Xyn10B.B2C were stable up to 60 °C. Xyn10B.C was stable only up to 50 °C as thermal unfolding was observed beyond these temperatures during CD spectroscopy analysis. All of the enzyme variants were highly active producing xylobiose and xylose as end products, as well as debranching the substrates by removing arabinose and acetyl side chains as observed by HPLC analysis of the lysates and arabinose/acetate assays. This study successfully elucidated the characteristcs of a novel trifunctional xylanase, Xyn10B. Due to its specific characteristics, Xyn10B.CB3B2 and its variants seem to be of importance for industrial applications. In another study, XynI from Caldicellulosiruptor saccharolyticus DSM 8903 was expressed in E. coli as 35.8 kDa protein in soluble form, but the expression level was rather low. MFOLD analysis of the sequence between the ribosomal binding site and the 5¢-end codons of the gene showed that the start codon AUG was trapped in the mRNA secondary structure. Cloning the gene using pET28a(+) increased expression to a level of 35% as compared to about 4% when expressed using pET22b(+). pET28a(+), having His-tag before the start codon, would prevent strong secondary structure formation thus allowing higher expression level. Activity of XynI was found to be 10, 5 and 6 U/mg on beechwood, birchwood and oat spelt xylan, respectively. Further studies are required to elucidate the reasons behind low activity through molecular modelling and docking analyses.
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