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مولانا محمد اویس نگرامی ندوی

مولانامحمد اویس نگرامی ندوی
افسوس ہے مولانا محمد اویس صاحب نگرامی بھی ایک طویل علالت کے بعد ؟اگست کی سہ پہر کولکھنؤ میں داعی اجل کولبیک کہہ کر اس خاکدانِ عالم سے رخصت ہوگئے۔ عمر ترسٹھ کے لگ بھگ ہوگی۔ اِنَّالِلّٰہِ وَاِنَّا اِلَیْہِ رَاجِعُوْن۔
نگرام لکھنؤ کاایک مردم خیز قصبہ ہے ،مولانا یہاں کے ایک نامور علمی خاندان کے چشم وچراغ تھے ۔تعلیم ندوہ میں پائی ،فراغت کے بعد دارالمصنفین اعظم گڈھ چلے گئے، کم وبیش سات برس یہاں مقیم رہ کر’ سیرت النبی‘ جلد اول پر نظرثانی کی۔ حافظ ابن قیم نے اپنی تصنیفات میں جہاں کہیں کسی آیت سے متعلق تفسیری کلام کیا ہے اُن سب کوتفسیر ابن قیم کے نام سے یکجا مرتب کیا،علاوہ ازیں معارف میں بھی متعدد مقالات لکھے ۔یوں توسب ہی علوم اسلامیہ میں پختہ استعداد رکھتے تھے لیکن قرآن مجید کاذوق سب پرغالب تھا۔ چنانچہ یہ سب مقالات بھی قرآن مجیدسے متعلق ہیں، دارالمصنفین سے جب وہ ندوۃ العلما میں منتقل ہوئے تویہاں بھی اُن کاخصوصی مشغلہ درسِ قرآن ہی رہا،مدرسہ کے اندر اوراُس کے باہر بھی۔ندوہ میں آنے کے بعد درس کی ہمہ گیر مصروفیتوں کے باعث وہ تصنیف وتالیف کی طرف زیادہ توجہ نہیں کرسکے،تاہم جوکچھ لکھ گئے ہیں اُس کی افادیت میں کلام نہیں ہوسکتا۔
طبعاً نہایت شگفتہ مزاج ،خوش خلق ،خوش پوشاک وخوش خوراک تھے، خندہ جبینی اُن کی فطرت تھی،عملاً نہایت صالح اور اوراد و وظائف تک کے پابند تھے۔ مولانا سیدحسین احمدصاحب مدنی ؒ سے بیعت تھے اور اس سلسلہ میں اُن سے برابر مراسلت بھی رکھتے تھے۔ اﷲ تعالیٰ غریقِ رحمت فرمائے۔
[اکتوبر ۱۹۷۶ء]

 

Effect of Service Experience and Perceived Value on Patients Satisfaction with Special Hospitals for Mom and Children

Patient satisfaction must be achieved in order to survive in the competitive hospital industry which tends to continue to grow beyond existing needs. This study aims to determine whether there is an effect of Service Experience and Perceived Value on patient satisfaction in the Inpatient Installation of the Regional Special Hospital of South Sulawesi Province (RSKD IA Pertiwi and RSKD IA Siti Fatimah). This type of research is a quantitative study using an observational study with a cross-sectional study design. The sample in this study were patients in the inpatient installation of the IA Pertiwi Regional Special Hospital (RSKD) and RSKD IA Siti Fatimah, totaling 130 respondents. The results showed that service experience based on functional clues variables, mechanic clues variables, humanistic clues variables, and perceived value affected patient satisfaction. It is recommended to every officer, both management and all employees, to maintain the dimensions of a good service experience, try to meet expectations, wants, and needs according to hospital standards, especially in the functional clues and humanistic clues dimensions by increasing the sensitivity of nurses in handling/care that can help complaints patients, there needs to be an increase in strategies in building customer value so that patients feel the pride felt by patients when they are treated in the hospital.

Establishment of Stable Cell Lines Expressing Proteins of Hcv Genotype 3A Pakistani Isolate and Their Molecular Characterization

Hepatitis C virus (HCV) infection is the leading cause of chronic hepatitis which progresses to hepatocellular carcinoma (HCC) afflicting > 170 million people worldwide. HCV 3a is the most common genotype (about 70% of all genotypes) circulating in Pakistan. Expression of HCV individual gene of 3a would facilitate therapeutic and vaccines strategies against chronic HCV and liver Cirrhosis. The aim of the present study was the establishment of stable Huh-7 cell lines expressing structural and non structural proteins of HCV Genotype 3a Pakistani isolate obtained from chronic HCV patients. Blood samples were obtained from chronic HCV-3a positive patients. HCV individual genes were amplified using PCR with gene specific primers having restriction sites and kozak sequence. These gene amplicons were cloned in TA and mammalian expression vector PcDNA3.1+. Restriction digestion analysis and sequencing results confirmed the successful cloning of HCV genes in PcDNA3.1 vector. Huh-7 cell lines were transfected with these constructed plasmids having structural or non-structural HCV genes in confluent cells with lipofectamine. Positive clones were selected with G418 and then confirmed by genome PCR. Subsequently, transcription and expression of the integrated genes were demonstrated by RT-PCR, sequencing and Western blot analysis. We successfully cloned and expressed seven HCV-3a genes in PcDNA3.1 mammalian expression vector. Results of western blot and sequencing PCR confirmed the stable expression of these seven genes. Various cytokines like Tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF- β), leptin and Angiotensin were used as indicator of disease progression in huh-7 cells stably expressing HCV structural and nonstructural proteins in vitro. Collectively, these observations suggest a significant involvement of HCV huh-7/Core, huh-7/E2 and huh-7/NS5A in the up-regulation of gene expression proinflammatory cytokine TNF-α and profibrotic TGF-β. No significant change in the expression of Angiotensin-II in vitro was observed. Up-regulation of leptin was observed in huh-7/E2 cell line only. The stable cell-lines expressing HCV-3a individual genes would be a useful tool to investigate the role of various HCV proteins on HCV disease outcome and testing of new therapeutic strategies against HCV.
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