Search from the Journals, Articles, and Headings
Advanced Search (Beta)
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

کراچی کا شیر

کراچی کا شیر

                                                                                                                مرزا مقبول احمد

کراچی کا شیر مگر جس کی بہادری اور جرات کے قصے پنجاب کی جیلوں شاہی قلعوں کے عقوبت خانوں میں لکھے ہوئے ہیں ۔دبلا پتلا سا خوبصورت جوان مرزا مقبول اپنے وزن سے بھاری بیڑیاں پہنے چھن چھن کرتا ہنستا مسکراتا کوٹ لکھپت جیل کے احاطے میں دندناتا پھرتا آج بھی نظروں میں گھوم جا تا ہے تو اس کے لیے ڈھیروں پیار دل میں بھر جا تا ہے ۔عارف لوہار کے چمٹے کی طرح بیڑیا ں بجاتے گنگناتے ہوئے :

جانے کیوں لوگ محبت کیا کرتے ہیں

دل کے بدلے درد دل لیا کرتے ہیں

گاتا تو محسوس ہو تا ہے کہ محبت کی کوئی ادھوری داستان جیل سے باہر چھوڑ آ یا ہے ۔مرزا مقبول استاد مسرور احسن ہو غلام حسین چانڈیو یا مصطفی میمن ہو یا دیگر ساتھی اپنی گہری یادیں پنجاب چھوڑ آئے ہیں، جنہیں دوست آج بھی یاد کرتے ہیں ہم بھول بھی کیسے سکتے ہیں اپنے انمول ساتھیوں کو ۔سندھ والو ان کی قدر کرو جس عزت و احترام کے قابل یہ لوگ ہیں انہیں ضرور دینا چاہیے ۔ایسے ہیروز روزر وز پیدا نہیں ہوتے ۔

 

 

Analysis of the Implementation of Patient Safety Targets at the Makassar City Regional General Hospital

The Patient Safety Goals (SKP) drive specific improvements in patient safety. These objectives highlight problematic areas of health care in a system implemented in hospitals to make patient care safer. This study aims to analyze the implementation of patient safety goals in Makassar City Hospital. This type of research is mixed methods research. The research uses a sequential explanatory strategy. The results showed that the implementation of patient safety targets based on the Hospital Patient Safety Target Standards (SNARS) at Makassar City Hospital has a good implementation of patient safety targets. The implementation of patient safety targets in terms of leadership in the Makassar City Regional General Hospital (RSUD), namely the awarding of awards has never been done, and supervision is carried out by looking at patient safety reports. In terms of human resources, training related to patient safety is still lacking and only during accreditation. Regarding policies, there are SOPs related to patient safety incidents and there is no clear sanction, only a warning. For teamwork, there is no availability of a patient safety team in the treatment room, only KMKP has a patient safety team. In addition, the implementation of patient safety goals in terms of communication, namely the existence of positive feedback given and followed up by the Patient Safety and Quality Committee (KMKP), as well as lack of socialization by KMKP, only at the time of accreditation.

Isolation and Evaluation of Phytochemicals from Mushrooms for the Inhibition of Hmg-Coa Reductase

Mushrooms have long been used as food and as a cure for many diseases since they form a low-caloric diet with great amount of proteins, vitamins and minerals. In particular, mushrooms are known to contain phytochemicals that could help in mitigating the development of many diseases such as cancer, diabetes and cardiovascular diseases. 3-hydroxy-3-methylglutaryl-CoA reductase is a first rate limiting enzyme in cholesterol biosynthesis. Inhibition of this enzyme is the prime target of drugs used in the treatment of high serum cholesterol levels in humans. Various synthetic drugs being used in controlling hypercholesterolemia are mostly ineffective in vivo and have high cost of production. Further cholesterol-induced oxidative stress has been considered as a major contributor in the development of atherosclerosis, thus the antioxidant activity of the selected mushrooms (Phellinus baumii, Trametes versicolor, Pleurotus osteratus and Agaricus bisporus) was determined by using DPPH (2, 2-diphenyl-1-picrylhydrazy), H2O2 (Hydrogen peroxide) radical scavenging assays and the total phenolic content was determined by using Folin-Ciocalteu method. The results revealed that the P. baumii exhibited significantly higher scavenging activity against free radicals as compared to other mushrooms under study. Moreover, only a few works have investigated the isolation of compounds from mushrooms that are responsible for lowering blood cholesterol levels. In this regard, this work tested the extracts of the selected mushrooms for their ability to inhibit recombinant HMG-CoA reductase isolated from Drosophila melanogaster. To this end, the cDNA-fragment encoding the 54- kDa catalytic domain of Drosophila HMG-CoA reductase was cloned into a modified pET30b (+) vector, named pET30f using the EcoRI and XhoI sites in the 1 2 multiple cloning site. Expression in this modified vector was under the control of a T7 promoter. The expressed recombinant HMG-CoA reductase was purified by using Ni-NTA chromatography and the molecular weight of the purified enzyme was determined to be 115 kDa by gel filtration chromatography. As a result, it was concluded that this catalytically active HMG-CoA reductase enzyme is a dimer. Moreover, kinetic analysis of this recombinant enzyme revealed the KM values of 0.2 ± 0.02 mM for HMG-CoA and 0.14 ± 0.01 mM for the cofactor NADPH. Extracts from the four evaluated mushroom species showed considerable HMGR inhibitory activity, with P. baumii extract being the most potent (Ki = 0.89 ± 0.18 μg/mL), so further investigations were done on this mushroom for the purification of the compounds responsible for inhibiting HMG-CoA reductase by using silica column chromatography and reverse phase semi-preparative-high performance liquid chromatography (HPLC). The masses and molecular formulas of the purified compounds were determined through Liquid chromatography mass spectrometry (LC-MS), Tandem mass spectrometry (MS-MS), Fourier transform ion cyclotron resonance (FT-ICR) and Gas Chromatography mass spectrometry (GC-MS). The isolated compounds in the most active peak from semi-preparative HPLC were Benzamine, 2,3,5,6-tetrachloro-4 methoxy (259 m/z) identified by using GC-MS while the other two compounds with m/z 399.2 and 457.26294 were detected by using LC-MS and FT-ICR respectively. Thus this study successfully demonstrated the use of catalytic domain of the Drosophila HMGR as a potential tool to identify the inhibitors of enzymatic activity. These preliminary studies on P. baumii have indicated the presence of potential compounds in this mushroom that may be helpful against hypercholesterolemia. So, further studies should be done on the structure elucidation of these compounds as well as isolation of other compounds 3 from P. baumii that may contribute to the development of novel therapeutic agents for lowering blood cholesterol levels.
Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.