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معافی دا پھل

معافی دا پھل

سکولاں وچ گرمی دیاں چھٹیاں سن، عادل اپنی دادی نوں ملن ملتان آیا ہویا سی۔ اوہ چھٹیاں وچ اپنی دادی کول ضرور آندا سی۔ ایس لئی کہ اوس دی دادی اوس نوںبہت پیار کر دے تے کئی شیواں لے کے دیندی سی۔ اک وار دادی نے اوس نوں آکھیا کہ میں بازار توں کجھ گھر دیاں شیواں خریدنیاں نیں۔ توں میرے نال بازار چل۔ عادل تیار ہو گیا۔ دونویں اک رکشے وچ بیٹھے تے بازار آ گئے۔

بازار وچ آ کے پہلاں اوہناں کجھ چیزاں دیاں قیمتاں پچھیاں تے فیر اک وڈے سٹور اندر چلے گئے۔ ایس سٹور تے پھل، سبزیاں، بسکٹ، مٹھائی کپڑے، بالاں دے کھڈوانے تے ضرورت دی ہر شے موجود سی۔ دادی خریداری وچ مصروف ہو گئی تے عادل اوتھے موجود شیواں نوں ویکھن لگ پیا۔ مٹھائی والے کائونٹر اگوں لنگھدے ہویاں۔ اوس دا مٹھائی کھاون نوں دل کیتا۔ اوس ادھر اُدھر ویکھیا۔ جدوں اوس نوں یقین ہو گیا کہ اوس نوں کوئی نئیں ویکھ رہیا تاں اوس اک گلاب جامن چک کے کھا لیا۔ جدوں اوس دی دادی مٹھائی والے کائونٹر کولوں لنگھی تاں عادل اوتھے ای کھڑا منہ ہلا رہیا سی تے شیرہ اوس دے منہ اتے لگا ہویا سی۔ اوس دی دادی فوراً اوس سٹور توں باہر لے کے آئی تے پچھیا کہ توں کیہ کھاہدا اے؟ اوس جواب دتا کجھ وی نئیں۔ دادی نے پچھیا کیہ توں مٹھائی کھاہدی اے؟ اوس جواب دتا جی دادی اماں، دادی اماں نے پچھیا کیہ ایس سٹور توں چک کے کھاہدی اے؟ اوس آکھیا جی ہاں۔ دادی نے پچھیا توں سٹور والے دی اجازت توں بغیر کھاہدی اے؟ اوس آکھیا جی ہاں، دادی نے آکھیا جا کے سٹور والے کولوں معافی منگ تے نالے پیسے ادا کر۔ عادل کیہندا اے کہ جے میں اوس نوں...

COVID-19 Vaccine Uptake in Pakistan: No Time to Err

COVID 19 pandemic has had a significant impact on social, physical, mental and financial aspects of human life. Among the sickness and despair experienced for last more than a year, COVID vaccination is a ray of hope. The uptake of COVID vaccines has remained low. The government, institutions as well healthcare professionals should take this responsibility of promoting vaccination. A strong will and simple nudges are what it takes to fight the menace of the COVID pandemic.

Identification of Nanoviruses in Banana from Pakistan and Possible Control Through Rnai

Banana Bunchy Top Virus (BBTV) is a member of genus Babuvirus of the family Nanoviridae, ssDNA virus transmitted by Pentalonia nigronervosa. Family Nanoviridae is divided into two genera: Nanovirus and Babuvirus. Nanovirus includes FBNYV, MDV, SCSV, while the genus Babuvirus include BBTV. In Pakistan, banana production is under severe loss due to BBTV. In the absence of natural resistance, the use of genetically engineered resistance is an attractive option. The main objective of this study was to develop resistance in banana against banana bunchy top virus through RNAi and the identification of unknown components of BBTV by a new technique called Rolling Circle Amplification (RCA). Rolling circle amplification (RCA) is a novel technique for the amplification of circular DNAs. This technique has been widely used for the amplification of geminiviruses but its use for the characterization of nanoviruses has not been reported. The identification of unknown component is also necessary to find out whether any additional component is associated with infectious unit or not. An analysis of the genetic diversity of BBTV was made by this valuable technique across Tando Jam, Sindh, Pakistan, to characterize components of banana bunchy top virus. The RCA product was digested with several restriction enzymes and was resolved in agarose gel. The resulting RFLP pattern resembled those expected for BBTV. In order to confirm the RFLP analysis, the DNA was probed with cloned components of BBTV. The probes for components DNA-S, DNA-N and DNA-M correctly hybridized to their respective fragment. We further cloned two components of BBTV to verify results. The cloned components were highly homologous to South Pacific group of BBTV as reported from Pakistan. The results of present studies confirmed that RCA technology can be used for characterization of nanoviruses. The technique is of great value to nanovirus research since the components that make up this group are still being discovered. This diversity (low) is also helpful in generating resistance against viruses. So, RNAi construct was made against MRep of BBTV to engineer resistance against BBTV. This construct was transiently checked in banana male flower bud. The buds agro-infiltrated with EHA105 gave better expression as compared to GV3101. Expression of BBTV genes from PVX and under 35S promoter was also observed. Expression of MRep and MP under PVX resulted in necrosis and cell death at the site of inoculation and severe leaf curling and necrosis in newly emerging vii leaves in MP. Clink, NSP and CP produced mild symptoms of leaf curling and mosaic, while CP produced necrotic response in inoculated leaves. When all these genes were expressed under 35S promoter in N. benthamiana 16c line, MP and Clink stabilized GFP specific mRNA and reduced GFP specific siRNA. MRep, NSP and CP did not show accumulation of GFP specific mRNA. These results identified that MP and Clink are supressors of silencing. The ability of MP to induce severe necrosis in inoculated and systemic leaves and RNA silencing suppressors indicates that MP is a major pathogenecity determinant in BBTV genome. Promoter regions of BBTV components may have application for heterologous transgene expression. Promoter regions of BBTV components were cloned in expression vector and checked it in N. benthamiana plants. Out of five components of BBTV, DNA-S, DNA-C and DNA-R did not show any GUS expression in N. benthamiana, while DNA-N showed some level of expression. The deletion of 200bp from 5’ end of DNA-N increased the promoter activity but was still low as compared to CaMV, 35S.
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