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دشتِ افسوس میں اک پھول کھلا ہو جیسے
دشتِ افسوس میں اک پھول کھلا ہو جیسے
تو خزاں زاد شجر سے ہی ملا ہو جیسے
ایک مدت سے بیابانی تھی دل میں میرے
تو مرے غم مرے ہر دکھ کا صلہ ہو جیسے
روح مجروح تھی اور ادھڑے تھے ٹانکے دل کے
تیرے آنے سے ہر اک زخم سلا ہو جیسے
تم سے بچھڑیں تو کسی طور بھی ہم جی نہ سکیں
سانس تو سانس ہے تم دل کی جلا ہو جیسے
تم فضاؔ چھائی ہو اک ابرِ کرم کی صورت
ہم نہ مل پائے یہی خود سے گلہ ہو جیسے
امام جلال الدین السیوطی رحمہ اللہ کی زندگی اور علم تصوف میں خدمات
The Almighty Allah has sent many intellectual and pious people after the Last Prophet and Messenger Allah Subhan-o-Tala for the security of Den-e-Islam. They not noly Protect Deen-e-Islam from the foreign imovation but take their roles in its world over spreading. One among these prestigious personalities and intellectual is Jalal Ud Din Asoyothi (849-911). He was a multi sided personality. At the same time he was a great scholar, Author, Reviewer and genius intellectual among his friends. You are the author of approximately 700 books. He has touched my aspect of life in written works. While having such a busy and prestigious life of honesty, truthfulness, rejecting the materialistic life having a heart for the spiritual life. He started to leads the life of mysticism and till death did not leave the life of mystics persons. In this mystic’s life he has written and review many books. Due to this mysticism he was serves the humanity and protect Deen-e-Islam from the false beliefs. In this research paper some of the aspect of mysticism in the light of his research works has been discussed.Epidemiology of Peste Des Petits Ruminants in Small Ruminants of Punjab
Among viral diseases , Peste des Petits Ruminants (PPR) is one of the main transboundary small ruminant diseases. PPR is an acute disease, highly contagious with clinical signs of high grade fever, occulonasal dischargea, necrotizing and erosive stomatitis, intestinal mucosal damage and pneumonia, with morbidity and mortality rate 100 and 90% respectively. Present study was designed to conduct seroepidemiological study of PPR virus in sheep and goat in the Punjab province. A total of 800 serum samples were collected from selected areas from sheep (n=400) and goat (n=400) from population of the selected areas. In the five districts including Faisalabad, Attock, Dera Ghazi Khan, Bhakkar, Kasur were sampled and the samples were divided proportionately based on population size of the respective districts. Results showed that disease frequency was higher during winter days and maximum seroprevalence was detected in December, followed by May and September the trend was observed to decline in the month of July. Different risk factors affecting prevalence of PPR were investigated by the help of Participatory epidemiological investigations. Further, with the help of molecular epidemiology and by using PPRV specific F gene the molecular nature of the virus was analyzed. Moreover, the efficacy and protective ability of PPR vaccines under field conditions were evaluated in sheep and goats. The first part of the study focused upon investigating the epidemiological parameters and its association with the PPR disease in Pakistan. The second part of my study focused to detect the anti-PPRV antibodies in sheep and goat population in Pakistan. The serum samples were subjected to c-ELISA to detect antibodies directed against the nucleoprotein of the Peste des Petits Ruminants (PPR) virus The overall seroprevalence calculated on the basis of c-ELISA was found to be61 % in sheep and 44% in goats although no significant difference was found between these two species. In the third phase specimen samples (Lymph nodes, Spleen, Nasal swabs) were collected by attending different out breaks in the selected districts, these samples were evaluated by RT-PCR. Sequencing and phylogentic analysis of the samples were performed. Prevalence of PPR virus detected by RT-PCR was 62.8 and 63.9% in sheep and goat, respectively. In present study difference between sheep and goat prevalence is non-significant. In the F gene phylogenetic relationship the sequence in study clustered with sequence pattern from Pak 09 with accession no. (FN996973.1), Bhutan 10 (FR667649.1) and Bangladesh 2000 (FR667556.1), branching pattern. In the present study maximum predilection site of the virus was found in lymph node (100%) followed by spleen (100%) and nasal swab (62.85%) from sheep. The goats of eastern Punjab districts showed maximum detection (100%) followed by southern Punjab district (88.88%). In the final phase of the study, antibody titre of sheep and goats were evaluated by using technique of indirect haemagglutination (IHA). Two vaccines were used in the trial containing Nigerian 75/1 strain used for commercial purpose vaccination in the country. A non-significant difference was present between two vaccine responses in present trial and both vaccines were equally effective. In conclusion the PPR prevalence ratio is increasing in sheep and goat population of Punjab. The F gene of PPRV can be used for the detection of PPR disease and lymph node is the prime organ for virus detection in sheep and goat.Journals by Discipline
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